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> Stratagene gradient 96 thermal cycler robocycler
Stratagene gradient 96 thermal cycler robocycler
Stratagene Gradient 96 Thermal Cycler Robocycler
The RoboCyclertemperature cycler is a microprocessor-controlled, fourblock
The system has four anodized aluminum blocks (see Figure 1), each
containing 96 precision-cut wells. The system is capable of holding
microfuge tubes or polypropylene plates. The unique four-block design
decreases cycling time by up to thirty percent and achieves well-to-well. Includes item as shown.
temperature uniformity of 0.1across the non-gradient hot blocks.1,2,3
The system is programmed via a keypad with a built-in beeper that alerts the
user when a key selection has been recognized. Data is displayed in a liquid
crystal display (LCD) (see Figure 2). Temperature and dwell time for each
block can be programmed through four sequentially linked windows.
? Blocks 1-3 can be programmed for temperatures between 25and
? Block 4, the cold block, can be programmed for a temperature range of
? Dwell times range from 1 second to 99 minutes, 59 seconds.
? Each window can be programmed to cycle up to 99 times.
? Each group of four windows (i.e., one program) can be stored
permanently in one of 99 program storage areas. Program information
may be easily reviewed or retrieved through a user-friendly display
? Up to ten programs can be linked together to run sequentially.
The Hot Top Assembly for the RoboCycler temperature cycler eliminates
the need to add an oil overlay to thin-wall reaction tubes by maintaining a
temperature that reduces the condensation formed in the tubes during
temperature cycling (See Appendix I).
If the temperature cycler is a gradient model,* Block 2, the special brass
gradient block, can be programmed for a temperature gradient within the
range of 45-99with a well-to-well temperature uniformity of 0.25.2 A linear
temperature gradient is created by heating one end of the brass block and
cooling the other end. For more information on the use of the gradient
FEATURES OF THE ROBOCYCLER TEMPERATURE CYCLER
Temperature Change Compensation
The RoboCycler temperature cycler automatically compensates for
temperature changes which can occur when most or all of the wells are used.
For example, when the arm moves tubes from the cold block (Block 4) to
the first hot block (Block 1) on the first cycle of the program, the
temperature of Block 1 may drop by as much as 4. The system
compensates by pausing the countdown and waiting momentarily at Block 1
until this block reheats to within a degree of the set point.
When the tubes move from Block 1 at, for example, 95, to Block 2 at
54, the temperature of Block 2 may rise as much as 3. On the first
cycle, the system will measure the temperature increase and adjust the set
point accordingly for future cycles for that block, in this case decreasing the
The thermoelectric Peltier cooling unit of Block 4 can be activated for preand
post-run sample cooling. When a preheat sequence is started, the unit
will begin cooling to the programmed temperature in the active window. If a
time has been programmed for the cold block, the unit will continue cooling
while running. Otherwise, the unit will shut off and begin cooling again with
30 minutes left in a program. When the program finishes, the block will be
at or near the programmed temperature of Block 4 in Window 4. At the end
of every program, the robotic arm will automatically place the tubes in the
cold block, which will remain at the set temperature until turned off.
The RoboCycler temperature cycler is designed to conform to international
safety specifications. It is equipped with an over-temperature cut-out device
in each of the three hot blocks. If a fault occurs which might cause a heater
to stay on, power is cut to the heater when it reaches a temperature of
approximately 110. The thermal cut-out device will then automatically
reset itself at approximately 90.
